Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystallographic structure of human zeta-crystallin is reported. Enzyme shows a tetrameric structure | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T53F | Tyr53Phe mutant displays a large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase. For 3-penten-2-one, 3-nonen-2-one, 2-hexenal and 4-hydroxy-2-hexenal mutant also shows a decrease in kcat | Homo sapiens |
T59F | Tyr59Phe mutant exhibits almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals,while the Km is increased for 4-hydroxy-2-hexenal | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | pH 7, 25°C, wild-type | Homo sapiens | |
0.015 | - |
NADPH | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.035 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.05 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.06 | - |
NADPH | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.06 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.06 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.08 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.13 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.13 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.13 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.482 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.535 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.55 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.86 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.94 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.26 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.45 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
2.3 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
5.45 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
6.12 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
8.6 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q08257 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hexenal + NADPH | - |
Homo sapiens | hexanal + NADP+ | - |
? | |
2-nonenal + NADPH | - |
Homo sapiens | nonanal + NADP+ | - |
? | |
2-pentenal + NADPH | - |
Homo sapiens | pentanal + NADP+ | - |
? | |
3-buten-2-one + NADPH | - |
Homo sapiens | 2-butanone + NADP+ | - |
? | |
3-nonen-2-one + NADPH | - |
Homo sapiens | 2-nonanone + NADP+ | - |
? | |
3-penten-2-one + NADPH | - |
Homo sapiens | 2-pentanone + NADP+ | - |
? | |
4-hydroxy-2-hexenal + NADPH | - |
Homo sapiens | 4-hydroxy-hexanal + NADP+ | - |
? | |
4-hydroxy-2-nonenal + NADPH | - |
Homo sapiens | 4-hydroxy-nonanal + NADP+ | - |
? | |
propenal + NADPH | - |
Homo sapiens | propanal + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration, 3 M NaCl | Homo sapiens |
homotetramer | gel filtration, 0.3 M NaCl | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
zeta-Crystallin | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.16 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.2 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.2 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.21 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.28 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.28 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.36 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.97 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
1.15 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.25 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.33 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.66 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
2.16 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
2.66 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
3.11 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
3.67 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
39.74 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
73.27 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.016 | - |
3-nonen-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.023 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.023 | - |
2-pentenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.025 | - |
3-penten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.051 | - |
2-hexenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.058 | - |
propenal | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.06 | - |
2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
0.13 | - |
3-buten-2-one | pH 7, 25°C, mutant T53F | Homo sapiens | |
0.23 | - |
2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
0.23 | - |
3-nonen-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
0.36 | - |
3-penten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
1.16 | - |
propenal | pH 7, 25°C, wild-type | Homo sapiens | |
1.5 | - |
propenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
1.66 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, mutant T59F | Homo sapiens | |
2.1 | - |
4-hydroxy-2-nonenal | pH 7, 25°C, wild-type | Homo sapiens | |
4.6 | - |
3-buten-2-one | pH 7, 25°C, mutant T59F | Homo sapiens | |
4.8 | - |
3-buten-2-one | pH 7, 25°C, wild-type | Homo sapiens | |
10.21 | - |
4-hydroxy-2-hexenal | pH 7, 25°C, wild-type | Homo sapiens |