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Literature summary for 1.6.5.5 extracted from

  • Porte, S.; Moeini, A.; Reche, I.; Shafqat, N.; Oppermann, U.; Farres, J.; Pares, X.
    Kinetic and structural evidence of the alkenal/one reductase specificity of human zeta-crystallin (2011), Cell. Mol. Life Sci., 68, 1065-1077.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallographic structure of human zeta-crystallin is reported. Enzyme shows a tetrameric structure Homo sapiens

Protein Variants

Protein Variants Comment Organism
T53F Tyr53Phe mutant displays a large increase in Km values for all substrates and the cofactor, but mainly towards 3-buten-2-one and propenal with a 30fold increase. For 3-penten-2-one, 3-nonen-2-one, 2-hexenal and 4-hydroxy-2-hexenal mutant also shows a decrease in kcat Homo sapiens
T59F Tyr59Phe mutant exhibits almost the same kinetic parameter values as the wild-type enzyme for 2-alkenals,while the Km is increased for 4-hydroxy-2-hexenal Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
NADPH pH 7, 25°C, wild-type Homo sapiens
0.015
-
NADPH pH 7, 25°C, mutant T59F Homo sapiens
0.035
-
3-buten-2-one pH 7, 25°C, wild-type Homo sapiens
0.05
-
3-nonen-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.06
-
NADPH pH 7, 25°C, mutant T53F Homo sapiens
0.06
-
3-penten-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.06
-
3-buten-2-one pH 7, 25°C, mutant T59F Homo sapiens
0.08
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T53F Homo sapiens
0.13
-
4-hydroxy-2-hexenal pH 7, 25°C, wild-type Homo sapiens
0.13
-
3-buten-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.13
-
2-hexenal pH 7, 25°C, mutant T53F Homo sapiens
0.482
-
2-nonenal pH 7, 25°C, wild-type Homo sapiens
0.535
-
3-penten-2-one pH 7, 25°C, wild-type Homo sapiens
0.55
-
4-hydroxy-2-nonenal pH 7, 25°C, wild-type Homo sapiens
0.86
-
propenal pH 7, 25°C, mutant T59F Homo sapiens
0.94
-
3-nonen-2-one pH 7, 25°C, wild-type Homo sapiens
1.26
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
1.45
-
propenal pH 7, 25°C, wild-type Homo sapiens
2.3
-
propenal pH 7, 25°C, mutant T53F Homo sapiens
5.45
-
2-hexenal pH 7, 25°C, wild-type Homo sapiens
6.12
-
2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
8.6
-
2-pentenal pH 7, 25°C, wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q08257
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hexenal + NADPH
-
Homo sapiens hexanal + NADP+
-
?
2-nonenal + NADPH
-
Homo sapiens nonanal + NADP+
-
?
2-pentenal + NADPH
-
Homo sapiens pentanal + NADP+
-
?
3-buten-2-one + NADPH
-
Homo sapiens 2-butanone + NADP+
-
?
3-nonen-2-one + NADPH
-
Homo sapiens 2-nonanone + NADP+
-
?
3-penten-2-one + NADPH
-
Homo sapiens 2-pentanone + NADP+
-
?
4-hydroxy-2-hexenal + NADPH
-
Homo sapiens 4-hydroxy-hexanal + NADP+
-
?
4-hydroxy-2-nonenal + NADPH
-
Homo sapiens 4-hydroxy-nonanal + NADP+
-
?
propenal + NADPH
-
Homo sapiens propanal + NADP+
-
?

Subunits

Subunits Comment Organism
dimer gel filtration, 3 M NaCl Homo sapiens
homotetramer gel filtration, 0.3 M NaCl Homo sapiens

Synonyms

Synonyms Comment Organism
zeta-Crystallin
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
2-nonenal pH 7, 25°C, wild-type Homo sapiens
0.16
-
3-buten-2-one pH 7, 25°C, wild-type Homo sapiens
0.2
-
3-penten-2-one pH 7, 25°C, wild-type Homo sapiens
0.2
-
2-pentenal pH 7, 25°C, wild-type Homo sapiens
0.21
-
3-nonen-2-one pH 7, 25°C, wild-type Homo sapiens
0.28
-
2-hexenal pH 7, 25°C, wild-type Homo sapiens
0.28
-
3-buten-2-one pH 7, 25°C, mutant T59F Homo sapiens
0.36
-
2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
0.97
-
3-buten-2-one pH 7, 25°C, mutant T53F Homo sapiens
1.15
-
4-hydroxy-2-nonenal pH 7, 25°C, wild-type Homo sapiens
1.25
-
propenal pH 7, 25°C, mutant T59F Homo sapiens
1.33
-
4-hydroxy-2-hexenal pH 7, 25°C, wild-type Homo sapiens
1.66
-
propenal pH 7, 25°C, wild-type Homo sapiens
2.16
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
2.66
-
3-penten-2-one pH 7, 25°C, mutant T53F Homo sapiens
3.11
-
3-nonen-2-one pH 7, 25°C, mutant T53F Homo sapiens
3.67
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T53F Homo sapiens
39.74
-
propenal pH 7, 25°C, mutant T53F Homo sapiens
73.27
-
2-hexenal pH 7, 25°C, mutant T53F Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0016
-
2-hexenal pH 7, 25°C, mutant T53F Homo sapiens
0.016
-
3-nonen-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.023
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T53F Homo sapiens
0.023
-
2-pentenal pH 7, 25°C, wild-type Homo sapiens
0.025
-
3-penten-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.051
-
2-hexenal pH 7, 25°C, wild-type Homo sapiens
0.058
-
propenal pH 7, 25°C, mutant T53F Homo sapiens
0.06
-
2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
0.13
-
3-buten-2-one pH 7, 25°C, mutant T53F Homo sapiens
0.23
-
2-nonenal pH 7, 25°C, wild-type Homo sapiens
0.23
-
3-nonen-2-one pH 7, 25°C, wild-type Homo sapiens
0.36
-
3-penten-2-one pH 7, 25°C, wild-type Homo sapiens
1.16
-
propenal pH 7, 25°C, wild-type Homo sapiens
1.5
-
propenal pH 7, 25°C, mutant T59F Homo sapiens
1.66
-
4-hydroxy-2-hexenal pH 7, 25°C, mutant T59F Homo sapiens
2.1
-
4-hydroxy-2-nonenal pH 7, 25°C, wild-type Homo sapiens
4.6
-
3-buten-2-one pH 7, 25°C, mutant T59F Homo sapiens
4.8
-
3-buten-2-one pH 7, 25°C, wild-type Homo sapiens
10.21
-
4-hydroxy-2-hexenal pH 7, 25°C, wild-type Homo sapiens