Application | Comment | Organism |
---|---|---|
synthesis | production of (6R)-levodione in Escherichia coli expressing the enzyme plus glucose dehydrogenase with a molar yield of 95% | Candida macedoniensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Candida macedoniensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42400 | - |
x * 45890, deduced from gene sequence, x * 42400, SDS-PAGE | Candida macedoniensis |
45890 | - |
x * 45890, deduced from gene sequence, x * 42400, SDS-PAGE | Candida macedoniensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida macedoniensis | - |
expression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketoisophorone + NADPH + H+ | i.e. 2,6,6-trimethyl-2-cyclohexen-1,4-dione | Candida macedoniensis | (6R)-levodione + NADP+ | i.e.2,6,6-trimethylcycolhexane-1,4-dione | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45890, deduced from gene sequence, x * 42400, SDS-PAGE | Candida macedoniensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Candida macedoniensis |