Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | alters the observed Mo(V) lineshape, mixed-type inhibitor, decreases both NADH:nitrate reductase and reduced methyl viologen:nitrate reductase activities | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
nitrate | at ionic strength of 50 mM | Spinacia oleracea | |
0.018 | - |
nitrate | at ionic strength of 200 mM | Spinacia oleracea | |
0.093 | - |
nitrate | in 5 mM phosphate buffer | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM MOPS, containing 0.01 mM FAD, 0.1 mM EDTA, pH 7.0, 50% glycerol, stable for several months | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH | - |
Spinacia oleracea | nitrite + NAD+ + H2O | - |
? | |
reduced methyl viologen + nitrate | - |
Spinacia oleracea | methyl viologen + nitrite | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
Cl- | binding to the native enzyme | Spinacia oleracea | |
176 | - |
Cl- | binding to the enzyme-NO3- complex | Spinacia oleracea |