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Literature summary for 1.7.1.1 extracted from

  • Campbell, W.H.
    Properties of bromophenol blue as an electron donor for higher plant NADH:nitrate reductase (1986), Plant Physiol., 82, 729-732.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
Bromophenol blue noncompetitive versus NADH Cucurbita sp.
p-hydroxymercuribenzoate inactivation of NADH:nitrate reductase activity, no loss of bromphenol blue: nitrate reductase activity Cucurbita sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 Bromophenol blue at 10 mM nitrate Cucurbita sp.
0.5
-
 nitrate at 0.2 mM bromophenol blue Cucurbita sp.

Organism

Organism UniProt Comment Textmining
Cucurbita sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bromophenol blue + nitrate
-
 Cucurbita sp. ? + nitrite
-
 ?
nitrate + NADH
-
 Cucurbita sp. nitrite + NAD+ + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 40 min, 50% loss of nitrate reductase activity, slight increase of bromphenol blue activity Cucurbita sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Cucurbita sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 Bromophenol blue
-
 Cucurbita sp.