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Literature summary for 1.7.1.1 extracted from

  • Provan, F.; Haavik, J.; Lillo, C.
    The regulatory phosphorylated serine in full-length nitrate reductase is necessary for optimal binding to a 14-3-3 protein (2006), Plant Sci., 170, 394-398.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana plumbaginifolia Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
S521D the protein is constitutively active under conditions where the wild type protein would be rapidly inactivated, such as in the dark Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
14-3-3 protein BMH1 0.0018 mM, reduces activity to less than 20%, at pH 6.0 Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ble Sepharose chromatography Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH
-
Nicotiana tabacum nitrite + NAD+ + H2O
-
?
nitrite + NADH
-
Nicotiana tabacum nitric oxide + NAD+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Nicotiana tabacum