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Literature summary for 1.7.1.10 extracted from

  • Wang, R.; Nicholas, D.J.D.
    Some properties of nitrite and hydroxylamine reductases from Derxia gummosa (1986), Phytochemistry, 25, 2463-2469.
No PubMed abstract available
Search for more literature for 1.7.1.10

Inhibitors

Inhibitors Comment Organism Structure
1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate 1.5 mM, 68% inhibition, hydroxylamine protects Derxia gummosa
2,2'-dipyridyl
-
 Derxia gummosa
8-hydroxyquinoline 0.1 mM, 50% inhibition of hydroxylamine reductase activity Derxia gummosa
Antimycin 1 0.1 mM, 26% inhibition of hydroxylamine reductase activity Derxia gummosa
dinitrophenol 0.1 mM, 80% inhibition of hydroxylamine reductase activity Derxia gummosa
Iodine 0.5 mM, 80% inhibition, preincubation with hydroxylamine protects Derxia gummosa
N-ethylmaleimide 0.1 mM, 58% inhibition of hydroxylamine reductase activity Derxia gummosa
NADH inhibits in absence of hydroxylamine Derxia gummosa
nitrite nitrite and hydroxylamine reductase activity are associated with the same enzyme, nitrite and hydroxylamine are competitive for the same binding site Derxia gummosa
p-chloromercuribenzoate 0.1 mM, 67% inhibition of hydroxylamine reductase activity Derxia gummosa
potassium cyanide
-
 Derxia gummosa
potassium thiocyanate 0.1 mM, 90% inhibition of hydroxylamine reductase activity, nitrite reductase activity is not inhibited Derxia gummosa
Rose bengal 0.5 mM, 94% inhibition Derxia gummosa
rotenone 0.1 mM, 52% inhibition of hydroxylamine reductase activity Derxia gummosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 NO2-
-
 Derxia gummosa
0.15
-
 NADH
-
 Derxia gummosa
5.3
-
 hydroxylamine
-
 Derxia gummosa

Organism

Organism UniProt Comment Textmining
Derxia gummosa
-
 nitrite and hydroxylamine reductase are associated within the same enzyme
-

Purification (Commentary)

Purification (Comment) Organism
nitrite and hydroxylamine reductase are associated with the same enzyme Derxia gummosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.422
-
 reduction of nitrite Derxia gummosa
0.68
-
 reduction of hydroxylamine Derxia gummosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + NADH 100% activity with NADH, 46% with NADPH, 117% with FADH2, 105% with FMNH2, 220% with methylviologen, 210% with benzyl viologen, 57% with Na2S2O4 Derxia gummosa NH3 + NAD+ + H2O
-
 ?
NO2- + NADH enzyme has both nitrite reductase and hydroxylamine reductase activity, main function may be nitrite reduction Derxia gummosa NH3 + H2O + NAD+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Derxia gummosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.52
-
 NO2-
-
 Derxia gummosa