General Stability | Organism |
---|---|
dithiothreitol stabilizes during storage | Rattus norvegicus |
freezing partially inactivates membrane bound enzyme | Rattus norvegicus |
NADH prevents heat damage | Rattus norvegicus |
solubilization with deoxycholate, n-butanol or acetone destroys membrane bound enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Rattus norvegicus | |
N-ethylmaleimide | not inhibited | Rattus norvegicus | |
p-chloromercuribenzoate | 0.05 mM, complete inhibition, subsequent addition of 3 mM cysteine restores the activity | Rattus norvegicus | |
phosphate | - |
Rattus norvegicus | |
Sodium arsenate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
hydroxylamine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | firmly attached to mitochondrial membrane | Rattus norvegicus | 16020 | - |
mitochondrion | firmly attached to membrane | Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | possible identity of hydroxylamine reductase and the enzyme which reduces anthranilic hydroxamic acid to the amide | Rattus norvegicus | NH3 + NAD+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
NADH prevents heat damage | Rattus norvegicus |
40 | - |
membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, no loss of activity | Rattus norvegicus |
50 | - |
membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, 50% loss of activity | Rattus norvegicus |
60 | - |
membrane bound enzyme, pH 7.4, dithiothreitol, complete loss of activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
complete loss of activity after brief exposure | Rattus norvegicus |
8.4 | - |
no loss of activity after brief exposure | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus |