Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
construction of homology model and docking studies of natural and non-natural substrates. Residues C190 and D197 play an essential role in the catalytic mechanism | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C190A | complete loss of activity | Escherichia coli |
D197N | complete loss of activity | Escherichia coli |
E230Q | about 4% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | Escherichia coli |
F228W | about 8% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | Escherichia coli |
H229A | about 15% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | Escherichia coli |
Q89A | poor activity | Escherichia coli |
Q89L | poor activity | Escherichia coli |
S90A | about 10% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | wild-type, pH 7.5, 25°C | Escherichia coli | |
0.0061 | - |
2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | wild-type, pH 7.5, 25°C | Escherichia coli | |
0.176 | - |
5-cyanopyrrolo[2,3-d]pyrimidin-4-one | wild-type, pH 7.5, 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ | Escherichia coli | natural substrate | 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q46920 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant E230Q, pH 7.5, 25°C | Escherichia coli |
3.8 | - |
substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant S90A, pH 7.5, 25°C | Escherichia coli |
4.4 | - |
substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant E230Q, pH 7.5, 25°C | Escherichia coli |
8.7 | - |
substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant F228W, pH 7.5, 25°C | Escherichia coli |
10.8 | - |
substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant F228W, pH 7.5, 25°C | Escherichia coli |
12.9 | - |
substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant S90A, pH 7.5, 25°C | Escherichia coli |
14.3 | - |
substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant H229A, pH 7.5, 25°C | Escherichia coli |
17.9 | - |
substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant H229A, pH 7.5, 25°C | Escherichia coli |
90 | - |
substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, wild-type, pH 7.5, 25°C | Escherichia coli |
117 | - |
substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, wild-type, pH 7.5, 25°C | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 1 year, 1% residual activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ | natural substrate | Escherichia coli | 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+ | - |
? | |
2-amino-5-cyanopyrrolo[2,3-d]pyrimidine + 2 NADPH + 2 H+ | analogue of natural substrate, poor substrate | Escherichia coli | 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidine + 2 NADP+ | - |
? | |
5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ | analogue of natural substrate | Escherichia coli | 5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
QueF | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
5-cyanopyrrolo[2,3-d]pyrimidin-4-one | wild-type, pH 7.5, 25°C | Escherichia coli | |
0.11 | - |
2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one | wild-type, pH 7.5, 25°C | Escherichia coli | |
0.142 | - |
NADPH | wild-type, pH 7.5, 25°C | Escherichia coli |