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Literature summary for 1.7.1.14 extracted from

  • Zhang, L.; Kudo, T.; Takaya, N.; Shoun, H.
    The B helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH (2002), J. Biol. Chem., 277, 33842-33847.
    View publication on PubMed
Search for more literature for 1.7.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 (DE3) cells Cutaneotrichosporon cutaneum
expressed in Escherichia coli JM109 (DE3) cells Fusarium oxysporum

Protein Variants

Protein Variants Comment Organism
G76A the mutation decreases the NADH-dependent activity to 40% of the wild type activity Fusarium oxysporum
K77A the mutation decreases the NADH-dependent activity Fusarium oxysporum
Q78A the mutation slightly improves the NADH-dependent activity Fusarium oxysporum
S73A the mutation decreases the NADH-dependent activity Fusarium oxysporum
S73G/S75G the double mutation significantly improves NADPH-dependent Nor activity without affecting, or even slightly increasing, the NADHd-ependent activity Fusarium oxysporum
S75A the mutation decreases the NADH-dependent activity Fusarium oxysporum
S75G the mutation significantly improves the NADPH-dependent Nor activity of isozyme Fnor, and the overall activity is accelerated via the NADPH-enhanced reduction step Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
-
-
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography Cutaneotrichosporon cutaneum
DEAE-cellulose column chromatography and Mono Q or Resource Q column chromatography Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NO + NADH + H+ isozyme Fnor utilizes only NADH as electron donor Fusarium oxysporum N2O + NAD+ + H2O
-
 ?
NO + NADH + H+ isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum N2O + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cytochrome P450nor
-
 Cutaneotrichosporon cutaneum
cytochrome P450nor
-
 Fusarium oxysporum
Fnor isozyme Fusarium oxysporum
NOR
-
 Cutaneotrichosporon cutaneum
NOR
-
 Fusarium oxysporum
P450nor
-
 Cutaneotrichosporon cutaneum
P450nor
-
 Fusarium oxysporum
Tnor isozyme Cutaneotrichosporon cutaneum

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cutaneotrichosporon cutaneum
heme
-
 Fusarium oxysporum
NADH isozyme Fnor utilizes only NADH as electron donor Fusarium oxysporum
NADH isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum
NADPH isozyme Tnor utilizes both NADH and NADPH as electron donors Cutaneotrichosporon cutaneum