Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
190000 | - |
SDS-PAGE | Triticale sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticale sp. | - |
Triticum sativum x Secale cereale, winter triticale | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and blue-dextran-Sepharose column chromatography | Triticale sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Triticale sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000013 | - |
crude extract, using FMNH as electron donor, in 50 mM Tris-HCl buffer, pH 7.4, at 25°C | Triticale sp. |
0.0000167 | - |
crude extract, using methyl viologen as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.7, at 25°C | Triticale sp. |
0.000117 | - |
crude extract, using NADPH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C | Triticale sp. |
0.000184 | - |
crude extract, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C | Triticale sp. |
0.455 | - |
after 2481.9fold purification, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C | Triticale sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + H+ + nitrate | - |
Triticale sp. | NAD(P)+ + nitrite + H2O | - |
? | |
reduced methyl viologen + nitrate | - |
Triticale sp. | oxidized methyl viologen + nitrite + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 190000, SDS-PAGE | Triticale sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | the enzyme is specific in relation to NADH as well as to NADPH although it shows lower specific activity in the presence of NADPH | Triticale sp. |