Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | elevated concentrations | Hordeum vulgare | |
NADPH | elevated concentrations | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Neurospora crassa | |
Molybdenum | - |
Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | Hordeum vulgare | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
L. cv. Steptoe nar1a and nar1a | - |
Hordeum vulgare | - |
nar7w genotypes | - |
Neurospora crassa | - |
nit-1 mutant | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Storage Stability | Organism |
---|---|
-80°C, extract, 6 months, without loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | - |
Hordeum vulgare | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | key enzyme in the assimilation path of nitrate to ammonium. Crude extracts possess endogenous NADPH regenerating systems capable of providing reducing equivalents for effective nitrate reduction in vitro | Hordeum vulgare | nitrite + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Neurospora crassa |
28 | - |
assay at | Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
quite stable for at least 24 h | Neurospora crassa |
10 | - |
quite stable for at least 24 h | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Neurospora crassa |
7.7 | - |
assay at | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Neurospora crassa | |
FAD | - |
Hordeum vulgare |