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Literature summary for 1.7.1.4 extracted from
- A triphosphopyridine nucleotide-specific nitrite reductase from Escherichia coli (1961), J. Biol. Chem., 236, 3330-3335.
General Stability
| General Stability | Organism |
|---|---|
| dialysis for 15 h against 1 mM cyanide, 1 mM 8-hydroxyquinoline or 1 mM EDTA followed by dialysis against 0.05 M phosphate buffer, no decrease in activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | 0.5 mM, 62% inhibition | Escherichia coli |  |
| CN- | 0.02 mM, 88% inhibition | Escherichia coli | |
| NADP+ | 0.06 mM, 13% inhibition, 0.27 mM, 43% inhibition | Escherichia coli | |
| p-chloromercuribenzoate | 0.001 mM, 83% inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
NO2- | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Escherichia coli | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli Bn | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + nitrite + H+ | specific for NADH | Escherichia coli | NAD(P)+ + NH3 + H2O | - |
? | |
| NAD(P)H + nitrite + H+ | specific for NADH | Escherichia coli Bn | NAD(P)+ + NH3 + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH 6.5: about 50% activity, pH 9.5: about 15% activity | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate | Escherichia coli | |
| NADPH | enzyme is specific for NADPH, NADH reaction rate approx. 1% of NADPH rate | Escherichia coli | |
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Release 2023.1 (January 2023)
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