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Literature summary for 1.7.1.6 extracted from
- Rat liver cytosolic azoreductase. Purification and characterization (1979), J. Biol. Chem., 254, 3930-3934.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | enzyme contains no FMN | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
NADPH | - |
Rattus norvegicus | |
| 0.32 | - |
Methyl red | - |
Rattus norvegicus | |
| 0.4 | - |
NADH | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Rattus norvegicus |
| 52000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.83 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-carboxy-4-N,N-dimethylazobenzene + NAD(P)H | trivial name methyl red | Rattus norvegicus | 2-carboxy-1-phenylamine + N,N-dimethyl-1,4-phenylenediamine + NADP+ | - |
? | |
| 4-(dimethylamino)azobenzene + 2 NADPH | specific for reduction of methyl red | Rattus norvegicus | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
| methyl red + 2 NADH + 2 H+ | - |
Rattus norvegicus | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
r | |
| methyl red + NADH + H+ | - |
Rattus norvegicus | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 25% activity, pH 7.0: about 20% activity | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one mol of enzyme contains 2 mol of FAD | Rattus norvegicus | |
| flavin | typical flavoprotein absorption spectrum | Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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