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Literature summary for 1.7.1.6 extracted from
- Purification of two azoreductases from Escherichia coli K12 (1993), Indian J. Exp. Biol., 31, 951-954.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
ponceau SX | azoreductase I | Escherichia coli | |
| 0.005 | - |
ponceau SX | azoreductase II | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
azoreductase II, gel filtration | Escherichia coli |
| 28000 | - |
2 * 28000, azoreductase I, SDS-PAGE | Escherichia coli |
| 53000 | - |
azoreductase I, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
azoreductases I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
azoreductase II, with tartrazine | Escherichia coli |
| 0.065 | - |
azoreductase II, with orange II | Escherichia coli |
| 0.07 | - |
azoreductase II, with amaranth | Escherichia coli |
| 0.112 | - |
azoreductase I, with orange II | Escherichia coli |
| 0.19 | - |
azoreductase I, with amaranth | Escherichia coli |
| 0.212 | - |
azoreductase I, with tartrazine | Escherichia coli |
| 0.315 | - |
azoreductase II, with ponceau SX | Escherichia coli |
| 0.32 | - |
azoreductase I, with ponceau SX | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(4-sulfo-1-naphthylazo)-2-naphthol-3,6-disulfonic acid + NADPH | - |
Escherichia coli | alpha-naphthylamine-4-sulfonic acid + beta-naphthol-3,6-disulfonic acid + NADP+ | - |
? | |
| 4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H | NADH is more effective | Escherichia coli | 4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
| Ponceaux SX + NAD(P)H | - |
Escherichia coli | ? | - |
? | |
| tartrazine + NAD(P)H | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28000, azoreductase I, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
azoreductases I and II, with ponceau SX | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
azoreductase I and II | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
| riboflavin | - |
Escherichia coli | |
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