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Literature summary for 1.7.1.6 extracted from

  • Blumel, S.; Knackmuss, H.J.; Stolz, A.
    Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F (2002), Appl. Environ. Microbiol., 68, 3948-3955.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.6

Application

Application Comment Organism
environmental protection potential for the treatment of azo dye contaminated wastewater Xenophilus azovorans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS using a phage T7-promotor system, no in vivo activity in recombinant strains due to missing azo dye uptake system Xenophilus azovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 gel filtration, differences in molecular weights are supposed to be due to an extraordinary behavior of the enzyme in gel filtration Xenophilus azovorans
30000
-
 SDS-PAGE Xenophilus azovorans
30280
-
 calculated from the deduced amino acid sequence Xenophilus azovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H Xenophilus azovorans substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H Xenophilus azovorans KF46F / DSM 13620 substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?

Organism

Organism UniProt Comment Textmining
Xenophilus azovorans Q8KU07 formerly Pseudomonas sp. KF46F
-
Xenophilus azovorans KF46F / DSM 13620 Q8KU07 formerly Pseudomonas sp. KF46F
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xenophilus azovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.8
-
 purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-2-naphthol and NADH as substrates Xenophilus azovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Xenophilus azovorans 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Xenophilus azovorans 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Xenophilus azovorans KF46F / DSM 13620 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Xenophilus azovorans KF46F / DSM 13620 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Xenophilus azovorans

Cofactor

Cofactor Comment Organism Structure
additional information flavin-free enzyme Xenophilus azovorans
NADH
-
 Xenophilus azovorans
NADPH preferred over NADH Xenophilus azovorans