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Literature summary for 1.7.1.6 extracted from

  • Liu, G.; Zhou, J.; Fu, Q.S.; Wang, J.
    The Escherichia coli azoreductase AzoR Is involved in resistance to thiol-specific stress caused by electrophilic quinones (2009), J. Bacteriol., 191, 6394-6400.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.6

Activating Compound

Activating Compound Comment Organism Structure
2-methylhydroquinone 0.5 mM, increase in expression of azoR mRNA relative to the expression at zero time before stress is applied, at 10 min 46.0, at 20 min 129.1 Escherichia coli
catechol 6 mM, increase in expression of azoR mRNA relative to the expression at zero time before stress is applied, at 10 min 15.6, at 20 min 31.9 Escherichia coli
Diamide 1 mM, increase in expression of azoR mRNA relative to the expression at zero time before stress is applied, at 10 min 11.4, at 20 min 8.1 Escherichia coli
H2O2 1 mM, increase in expression of azoR mRNA relative to the expression at zero time before stress is applied, at 10 min 0.9, at 20 min 0.6, 10 mM, 10 min 3.0, 20 min 1.9 Escherichia coli
menadione 0.3 mM, increase in expression of azoR mRNA relative to the expression at zero time before stress is applied, at 10 min 25.5, at 20 min 13.3 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX-4T-1 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0081
-
 9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
 Escherichia coli
0.0146
-
 9,10-dioxo-9,10-dihydroanthracene-2,6-disulfonic acid
-
 Escherichia coli
0.0873
-
 2-Hydroxy-1,4-naphthoquinone
-
 Escherichia coli
1.704
-
 2-methyl-1,4-benzoquinone
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P41407
-
-

Purification (Commentary)

Purification (Comment) Organism
the GST-AzoR protein is purified by glutathione Sepharose 4B, the GST tag is removed by digestion with thrombin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-1,4-naphthoquinone + NADH + H+
-
 Escherichia coli ?
-
 ?
2-methyl-1,4-benzoquinone + NADH + H+
-
 Escherichia coli ?
-
 ?
anthraquinone-2,6-disulfonate + NADH + H+
-
 Escherichia coli ?
-
 ?
anthraquinone-2-sulfonate + NADH + H+
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
AzoR
-
 Escherichia coli
azoreductase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
 Escherichia coli
2.6
-
 9,10-dioxo-9,10-dihydroanthracene-2,6-disulfonic acid
-
 Escherichia coli
5.8
-
 2-Hydroxy-1,4-naphthoquinone
-
 Escherichia coli
72
-
 2-methyl-1,4-benzoquinone
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity assay Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Escherichia coli
NADH
-
 Escherichia coli