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Literature summary for 1.7.1.6 extracted from

  • Punj, S.; John, G.
    Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis (2009), Curr. Issues Mol. Biol., 11, 59-66.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
Methyl red in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
0.082
-
NADH in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
15
-
NADPH in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis ATCC 27274
-
-
-

Purification (Commentary)

Purification (Comment) Organism
octyl Sepharose column chromatography, DEAE Bio-gel agarose column chromatography, and Affi-gel Blue gel filtration Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
crude extract, using methyl red and NADPH as substrates, in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
626
-
after 209fold purification, using methyl red and NADPH as substrates, in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red + NADH + H+
-
Enterococcus faecalis ?
-
?
methyl red + NADH + H+
-
Enterococcus faecalis ATCC 27274 ?
-
?
methyl red + NADPH + H+
-
Enterococcus faecalis ?
-
?
methyl red + NADPH + H+
-
Enterococcus faecalis ATCC 27274 ?
-
?

Synonyms

Synonyms Comment Organism
AzoA
-
Enterococcus faecalis
FMN-dependent NAD(P)H azoreductase
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
FMN dependent on Enterococcus faecalis
NADH AzoA shows more than 180fold preference for NADH over NADPH as an electron donor to reduce methyl red Enterococcus faecalis
NADPH AzoA shows more than 180fold preference for NADH over NADPH as an electron donor to reduce methyl red Enterococcus faecalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0019
-
Methyl red in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis