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Literature summary for 1.7.1.6 extracted from
- Identification, Isolation and characterization of a novel azoreductase from Clostridium perfringens (2012), Anaerobe, 18, 229-234.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Esacherichia coli | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxygen | presence of oxygen in the assay reduces the activity | Clostridium perfringens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00018 | - |
FAD | pH not specified in the publication, temperature not specified in the publication | Clostridium perfringens | |
| 0.005 | - |
Direct blue 15 | pH not specified in the publication, temperature not specified in the publication | Clostridium perfringens | |
| 0.0081 | - |
NADH | pH not specified in the publication, temperature not specified in the publication | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22600 | - |
4 * 22600, SDS-PAGE | Clostridium perfringens |
| 90400 | - |
gel filtration | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| direct blue 15 + FAD + NADH + H+ | highest activity in presence of both NADH and FAD | Clostridium perfringens | ? + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 22600, SDS-PAGE | Clostridium perfringens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AzoC | - |
Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Clostridium perfringens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Clostridium perfringens | |
| NADH | - |
Clostridium perfringens | |
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Release 2023.1 (January 2023)
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