KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Methyl red | cofactor roseoflavin mononucleotide, pH 7.4, 30°C | Escherichia coli | |
0.027 | - |
Methyl red | cofactor FMN, pH 7.4, 30°C | Escherichia coli | |
0.26 | - |
NADH | cofactor FMN, pH 7.4, 30°C | Escherichia coli | |
0.269 | - |
NADH | cofactor roseoflavin mononucleotide, pH 7.4, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl red + NADH + H+ | - |
Escherichia coli | ? + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli | |
additional information | the flavin mononucleotide analogue roseoflavin mononucleotide binds to the AzoR apoenzyme with an even higher affinity compared to that of the natural cofactor FMN. Roseoflavin mononucleotide binding does not affect the overall topology of the enzyme and also does not interfere with dimerization of AzoR. The AzoR-roseoflavin mononucleotide holoenzyme complex shows 30% of AzoR-FMN activity in a standard assay | Escherichia coli |