Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3) cells | Halomonas elongata |
Protein Variants | Comment | Organism |
---|---|---|
L49C/D108C | the mutant enzyme shows increased thermal stability at 50°C (increased half-life from 12.6 min in wild type to 26.66 min in the mutant enzyme). The mutant enzyme can also tolerate 5% (w/v) NaCl and retains 30% of original activity after 24 h incubation | Halomonas elongata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | the wild type enzyme is completely inactivated by 5% (w/v) NaCl after 24 h incubation | Halomonas elongata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Methyl red | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata | |
0.044 | - |
Methyl red | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata | |
0.091 | - |
NADH | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata | |
0.223 | - |
NADH | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Halomonas elongata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas elongata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Halomonas elongata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl red + NADH | - |
Halomonas elongata | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22000, SDS-PAGE | Halomonas elongata |
Synonyms | Comment | Organism |
---|---|---|
AzoH | - |
Halomonas elongata |
azoreductase | - |
Halomonas elongata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of 12.6 min at 50°C | Halomonas elongata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADH | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata | |
0.048 | - |
Methyl red | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata | |
0.148 | - |
NADH | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata | |
0.148 | - |
Methyl red | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Halomonas elongata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
NADH | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata | |
1.01 | - |
Methyl red | mutant enzyme L49C/D108C, at pH 7.6 and 25°C | Halomonas elongata | |
1.62 | - |
NADH | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata | |
11.38 | - |
Methyl red | wild type enzyme, at pH 7.6 and 25°C | Halomonas elongata |