Literature summary for 1.7.1.7 extracted from

Renart, M.F.; Sillero, A.
GMP reductase in Artemia salina (1974), Biochim. Biophys. Acta, 341, 178-186.

Search for more literature for 1.7.1.7

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	in absence of mercaptoethanol 50% of maximal velocity	Artemia salina

Inhibitors

Inhibitors	Comment	Organism	Structure
IMP	1 mM, 10% inhibition, 2 mM, 20% inhibition	Artemia salina
additional information	not inhibited by ATP	Artemia salina
p-hydroxymercuribenzoate	-	Artemia salina

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	GMP	-	Artemia salina

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
250000	-	gel filtration	Artemia salina

Organism

Organism	UniProt	Comment	Textmining
Artemia salina	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Artemia salina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.078	-	-	Artemia salina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADPH + guanosine 5'-phosphate	the rate of the reverse reaction is 6% of the forward reaction	Artemia salina	NADP+ + inosine 5'-phosphate + NH3	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Artemia salina

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9.5	pH 7: about 25% of activity at maximum, pH 9.5: about 30% of activity at maximum	Artemia salina

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Artemia salina
NADPH	specific for	Artemia salina

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.035	-	IMP	-	Artemia salina

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)