Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM required for maximal activity | Klebsiella aerogenes | |
cysteine | 2 mM required for maximal activity | Klebsiella aerogenes | |
GSH | 2 mM required for maximal activity | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Chloro-9-beta-D-ribofuranosylpurine 5'-phosphate | 0.01 mM, 80% inhibition after 40 min, 1 mM GMP protect | Klebsiella aerogenes | |
6-Thio-GMP | 0.009 mM, 81% inhibition after 30 min | Klebsiella aerogenes | |
6-thio-IMP | 0.32 mM, 75% inhibition after 15 min | Klebsiella aerogenes | |
adenosine 2'-phosphate | - |
Klebsiella aerogenes | |
ADP | 2 mM, 85% inhibition | Klebsiella aerogenes | |
AMP | - |
Klebsiella aerogenes | |
ATP | 0.4 mM, 92% inhibition | Klebsiella aerogenes | |
GTP | slight | Klebsiella aerogenes | |
IMP | - |
Klebsiella aerogenes | |
iodoacetamide | progressive inhibition, 2 mM GMP protect | Klebsiella aerogenes | |
iodoacetate | progressive inhibition, 2 mM GMP protect | Klebsiella aerogenes | |
p-mercuribenzoate | 2 mM, complete inhibition, complete protection by 2 mM GSH | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
212.0 units/mg, 1 unit is defined as the amount of enzyme giving an optical density change of 1.00/min | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + guanosine 5'-phosphate | - |
Klebsiella aerogenes | NADP+ + inosine 5'-phosphate + NH3 | - |
? |