Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldithiocarbamate | 1 mM, complete inhibition | Haloarcula marismortui | |
EDTA | 1 mM, 70% inhibition after 1 h | Haloarcula marismortui | |
KCN | 1 mM, complete inhibition | Haloarcula marismortui |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | blue copper enzyme, 1 type 1 and 1 type 2 Cu atoms per subunit | Haloarcula marismortui | |
copper | green copper enzyme | Haloferax denitrificans | |
copper | green copper enzyme | Achromobacter cycloclastes | |
copper | blue copper enzyme | Fusarium oxysporum | |
copper | blue copper enzyme | Achromobacter xylosoxidans | |
KCl | 2 M, enzyme is activated by high salt concentrations | Haloarcula marismortui | |
LiCl | 2 M, enzyme is activated by high salt concentrations | Haloarcula marismortui | |
NaNO3 | 2 M, enzyme is activated by high salt concentrations | Haloarcula marismortui | |
NH4Cl | 2 M, enzyme is activated by high salt concentrations | Haloarcula marismortui |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
3 * 36500, calculated from sequence, crystal structure | Achromobacter xylosoxidans |
37000 | - |
3 * 37000, calculated from sequence, crystal structure | Achromobacter cycloclastes |
41800 | - |
2 * 41800, SDS-PAGE | Fusarium oxysporum |
46000 | - |
3 * 46000, SDS-PAGE | Haloarcula marismortui |
64000 | - |
2 * 64000, SDS-PAGE | Haloferax denitrificans |
69000 | - |
gel filtration | Achromobacter cycloclastes |
70000 | - |
gel filtration | Achromobacter xylosoxidans |
83300 | - |
gel filtration | Fusarium oxysporum |
103000 | - |
sedimentation equilibrium | Achromobacter xylosoxidans |
105000 | - |
sedimentation equlibrium | Achromobacter cycloclastes |
127000 | - |
gel filtration | Haloferax denitrificans |
167000 | - |
gel filtration | Haloarcula marismortui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter cycloclastes | P25006 | - |
- |
Achromobacter xylosoxidans | - |
- |
- |
Fusarium oxysporum | - |
- |
- |
Haloarcula marismortui | - |
- |
- |
Haloferax denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
butyl-toyopearl, Sepharose CL-6B, Sephacryl S-300, octyl-sepharose, hydroxyapatite | Haloarcula marismortui |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
960 | - |
in the presence of 2 M NaCl | Haloarcula marismortui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + ferrocytochrome c | - |
Haloarcula marismortui | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c | - |
Fusarium oxysporum | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c | - |
Achromobacter xylosoxidans | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c | - |
Haloferax denitrificans | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c | - |
Achromobacter cycloclastes | nitric oxide + H2O + ferricytochrome c | - |
? | |
NO2- + Na2S2O4 | pysiological electron donor is unknown, no activity with methyl viologen, phenazine methosulfate or N,N,N',N',-tetramethyl-p-phenylenediamine | Haloarcula marismortui | NO + Na2S2O3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 64000, SDS-PAGE | Haloferax denitrificans |
dimer | 2 * 41800, SDS-PAGE | Fusarium oxysporum |
trimer | 3 * 46000, SDS-PAGE | Haloarcula marismortui |
trimer | 3 * 37000, calculated from sequence, crystal structure | Achromobacter cycloclastes |
trimer | 3 * 36500, calculated from sequence, crystal structure | Achromobacter xylosoxidans |