Protein Variants | Comment | Organism |
---|---|---|
additional information | anaerobically grown Escherichia coli nrf mutants are more sensitive to NO radicals than the parent strain | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | cyanide is a potent inhibitor of NrfA nitrite and hydroxylamine reductase activities | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
nitrite | pH 7.0, 20°C, reduction | Escherichia coli | |
0.3 | - |
NO radical | pH 7.0, 20°C, reduction | Escherichia coli | |
60.4 | - |
hydroxylamine | pH 7.0, 20°C, reduction | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | NrfA from Escherichia coli has a well established role in the respiratory reduction of nitrite to ammonium, it might also participate in NO radical detoxification, detoxifying exogenously generated NO radical encountered during invasion of a human host, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
quantitation of steady-state NrfA reduction of NO radical, nitrite, and hydroxylamine | Escherichia coli |
25 | - |
nitrite reduction | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + reduced methyl viologen | low activity | Escherichia coli | ? | - |
? | |
additional information | NrfA from Escherichia coli has a well established role in the respiratory reduction of nitrite to ammonium, it might also participate in NO radical detoxification, detoxifying exogenously generated NO radical encountered during invasion of a human host, overview | Escherichia coli | ? | - |
? | |
additional information | the enzyme shows a common site for reduction of all three substrates as axial ligands to the lysine-coordinated NrfA heme rather than nonspecific NO radical reduction at one of the four His-His coordinated hemes also present in each NrfA subunit. NO radical reduction is initiated at similar potentials to NrfA-catalyzed reduction of nitrite and hydroxylamine | Escherichia coli | ? | - |
? | |
nitrite + reduced methyl viologen | - |
Escherichia coli | NH3 + H2O + oxidized methyl viologen | - |
? | |
NO radical + reduced methyl viologen | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome c nitrite reductase | - |
Escherichia coli |
NrfA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 25 | NrfA activity increases when the temperature is raised from 4°C to 25°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
700 | - |
nitrite | pH 7.0, 20°C, reduction | Escherichia coli | |
840 | - |
NO radical | pH 7.0, 20°C, reduction | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH dependence of NrfA at 4-25°C, overview | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli | |
methyl viologen | used as the electron donor, reduced with dithionite, artificial cofactor | Escherichia coli |