Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | slight | Escherichia coli | |
EDTA | 39% inhibition at 10 mM | Escherichia coli | |
KCN | 71% inhibition at 10 mM | Escherichia coli | |
NaN3 | 39% inhibition at 10 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
oxidized methyl viologen | with trimethylamine N-oxide, pH 6.9 | Escherichia coli | |
0.33 | - |
Oxidized benzyl viologen | with trimethylamine N-oxide | Escherichia coli | |
0.67 | - |
Trimethylamine-N-oxide | with methyl viologen, pH 6.9 | Escherichia coli | |
0.95 | - |
Trimethylamine-N-oxide | with benzyl viologen, pH 5.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains 0.96 atoms of iron | Escherichia coli | |
Molybdenum | enzyme contains 1.96 atoms of molybdenum | Escherichia coli | |
Molybdenum | contains molybdopterin | Escherichia coli | |
Zinc | enzyme contains 1.52 atoms of zinc | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
2 * 95000, SDS-PAGE | Escherichia coli |
200000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
TMAO reductase I, the major enzyme among inducible TMAO reductases | - |
Purification (Comment) | Organism |
---|---|
using heat treatment, ammonium sulfate precipitation and chromatography on Bio-Gel A-1.5m, DEAE-cellulose and reactive blue-agarose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1620 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-picoline N-oxide + electron donor | - |
Escherichia coli | alpha-picoline + H2O + oxidized electron donor | - |
? | |
chlorate + electron donor | - |
Escherichia coli | ? | - |
? | |
hydroxylamine N-oxide + electron donor | - |
Escherichia coli | hydroxylamine + H2O + oxidized electron donor | - |
? | |
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | NADH as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + oxidized benzyl viologen + H+ | - |
Escherichia coli | trimethylamine + reduced benzyl viologen + H2O | - |
r | |
trimethylamine N-oxide + oxidized methyl viologen + H+ | - |
Escherichia coli | trimethylamine + reduced methyl viologen + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 95000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, enzyme activity does not change | Escherichia coli |
75 | - |
60% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
with benzyl viologen | Escherichia coli |
6.9 | - |
with methyl viologen | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains more than 0.4 atoms of acid-labile sulfur per molecular weight of 200000 | Escherichia coli | |
NADH | - |
Escherichia coli |