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Literature summary for 1.7.2.3 extracted from

  • Sajitz, P.; Klemme, J.; Koch, H.; Molitor, M.
    Isolation and properties of trimethylamine N-oxide/dimethylsulfoxide reductase from the purple bacterium Rhodospirillum rubrum (1993), Z. Naturforsch. C, 48, 812-814.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
Dimethylsulfoxide
-
Rhodospirillum rubrum
24
-
Trimethylamine-N-oxide
-
Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Rhodospirillum rubrum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum deduced from inhibition of enzyme synthesis by tungstate and reduction of enzyme levels in charcoal-treated media Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
1 * 40000 + 1 * 36000, SDS-PAGE Rhodospirillum rubrum
40000
-
1 * 40000 + 1 * 36000, SDS-PAGE Rhodospirillum rubrum
83000
-
gel electrophoresis Rhodospirillum rubrum
87000
-
gel filtration Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate treatment and chromatography on Sephacryl S-200 column and Mono-Q column Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
periplasmic fraction Rhodospirillum rubrum
10.2
-
purified enzyme Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylsulfoxide + electron donor 9% relative activity to trimethylamine N-oxide Rhodospirillum rubrum ? + oxidized electron donor + H2O
-
?
L-methionine sulfoxide + electron donor 26% relative activity to trimethylamine N-oxide Rhodospirillum rubrum L-methionine + oxidized electron donor + H2O
-
?
additional information nitrate does not act as electron acceptor Rhodospirillum rubrum ?
-
?
trimethylamine N-oxide + electron donor benzyl viologen as electron donor Rhodospirillum rubrum trimethylamine + oxidized electron donor + H2O
-
?

Subunits

Subunits Comment Organism
dimer 1 * 40000 + 1 * 36000, SDS-PAGE Rhodospirillum rubrum