KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
Dimethylsulfoxide | - |
Rhodospirillum rubrum | |
24 | - |
Trimethylamine-N-oxide | - |
Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Rhodospirillum rubrum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | deduced from inhibition of enzyme synthesis by tungstate and reduction of enzyme levels in charcoal-treated media | Rhodospirillum rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 40000 + 1 * 36000, SDS-PAGE | Rhodospirillum rubrum |
40000 | - |
1 * 40000 + 1 * 36000, SDS-PAGE | Rhodospirillum rubrum |
83000 | - |
gel electrophoresis | Rhodospirillum rubrum |
87000 | - |
gel filtration | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate treatment and chromatography on Sephacryl S-200 column and Mono-Q column | Rhodospirillum rubrum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
periplasmic fraction | Rhodospirillum rubrum |
10.2 | - |
purified enzyme | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylsulfoxide + electron donor | 9% relative activity to trimethylamine N-oxide | Rhodospirillum rubrum | ? + oxidized electron donor + H2O | - |
? | |
L-methionine sulfoxide + electron donor | 26% relative activity to trimethylamine N-oxide | Rhodospirillum rubrum | L-methionine + oxidized electron donor + H2O | - |
? | |
additional information | nitrate does not act as electron acceptor | Rhodospirillum rubrum | ? | - |
? | |
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Rhodospirillum rubrum | trimethylamine + oxidized electron donor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 40000 + 1 * 36000, SDS-PAGE | Rhodospirillum rubrum |