Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant lacking a protein, TorD, which is a chaperone specific for the enzyme | - |
Purification (Comment) | Organism |
---|---|
using ion exchange chromatography on DE52, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
periplasmic fraction of wild-type cells, initial activity | Escherichia coli |
250 | - |
purified enzyme | Escherichia coli |
1350 | - |
crude extract of mutant strain lacking the TorD protein | Escherichia coli |
1720 | - |
crude extract of wild-type strain | Escherichia coli |
1810 | - |
crude extract of mutant strain lacking the TorD protein but complemented by pTorD | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | quinone as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? |