General Stability | Organism |
---|---|
turnover dependent inactivation, promoted by zinc ions at 0.01 mM | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | turnover dependent inactivation | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66180 | - |
2 * 66180, MALDI-TOF | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | Cereibacter sphaeroides | - |
nitrogen + H2O + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
f. sp. denitrificans IL106 | - |
Purification (Comment) | Organism |
---|---|
purification under anaerobic conditions | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44 | - |
aerobic form | Cereibacter sphaeroides |
78 | - |
anaerobic form | Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
4°C, retained most of its activity after aerobic storage for 2 months without any additives | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | - |
Cereibacter sphaeroides | nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | dithionite-reduced benzyl viologen as electron donor | Cereibacter sphaeroides | nitrogen + H2O + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 66180, MALDI-TOF | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
most of activity lost | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | with reduced benzyl viologen as electron donor | Cereibacter sphaeroides |