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Literature summary for 1.7.2.4 extracted from
- Biochemical characterization and solution structure of nitrous oxide reductase from Alcaligenes xylosoxidans (NCIMB 11015) (1999), Eur. J. Biochem., 259, 651-659.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | contains 7.1 atoms per dimer | Achromobacter xylosoxidans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66871 | - |
2 * 66871, ESI mass spectrometry | Achromobacter xylosoxidans |
| 134000 | - |
gel filtration | Achromobacter xylosoxidans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | Achromobacter xylosoxidans | - |
nitrogen + H2O + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter xylosoxidans | - |
NCIMB 11015, X-ray scattering data, 20 A resolution | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification under anaerobic conditions | Achromobacter xylosoxidans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Achromobacter xylosoxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | - |
Achromobacter xylosoxidans | nitrogen + H2O + acceptor | - |
? | |
| nitrous oxide + reduced acceptor | dithionite-reduced methyl viologen as electron donor | Achromobacter xylosoxidans | nitrogen + H2O + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 66871, ESI mass spectrometry | Achromobacter xylosoxidans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 85 | activity is maximal after exposure to high temperatures | Achromobacter xylosoxidans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | activity is maximal after incubation at high pH values | Achromobacter xylosoxidans |
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