Cloned (Comment) | Organism |
---|---|
the gene encoding the enzyme in the nos cluster is cloned from a genomic library | Achromobacter cycloclastes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Achromobacter cycloclastes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme contains average 4.5 Cu and 1.2 S per monomer | Achromobacter cycloclastes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced methyl viologen | Achromobacter cycloclastes | - |
nitrogen + H2O + methyl viologen | - |
? | |
nitrous oxide + reduced methyl viologen | Achromobacter cycloclastes IAM1013 | - |
nitrogen + H2O + methyl viologen | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter cycloclastes | P94127 | - |
- |
Achromobacter cycloclastes IAM1013 | P94127 | - |
- |
Purification (Comment) | Organism |
---|---|
anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration | Achromobacter cycloclastes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
124 | - |
purified enzyme, pH 8.0, 25°C | Achromobacter cycloclastes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced methyl viologen | - |
Achromobacter cycloclastes | nitrogen + H2O + methyl viologen | - |
? | |
nitrous oxide + reduced methyl viologen | - |
Achromobacter cycloclastes IAM1013 | nitrogen + H2O + methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered | Achromobacter cycloclastes |
Synonyms | Comment | Organism |
---|---|---|
N2OR | - |
Achromobacter cycloclastes |
nitrous oxide reductase | - |
Achromobacter cycloclastes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Achromobacter cycloclastes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 8 | assay at | Achromobacter cycloclastes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 10.6 | perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered | Achromobacter cycloclastes |