Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum aerophilum |
Crystallization (Comment) | Organism |
---|---|
modeling of structure. The residues contributing to the semiocclusion of the T1 copper site are Trp355, Met389, and Met297. There is a negatively charged residue in the neighborhood of the T1 site, Glu296 | Pyrobaculum aerophilum |
Protein Variants | Comment | Organism |
---|---|---|
E296Q | mutation near T1 copper site, similar biochemical and spectroscopic properties to those of the wild type | Pyrobaculum aerophilum |
M297A | mutation near T1 copper site, similar biochemical and spectroscopic properties to those of the wild type | Pyrobaculum aerophilum |
M389A | mutation near T1 copper site, similar biochemical and spectroscopic properties to those of the wild type | Pyrobaculum aerophilum |
W355A | mutation near T1 copper site, similar biochemical and spectroscopic properties to those of the wild type | Pyrobaculum aerophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
N2O | pH 7.6, 25°C | Pyrobaculum aerophilum | |
0.033 | - |
Fe2+ | pH 7.6, 25°C | Pyrobaculum aerophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 3.2 mol of copper per mol of enzyme, in the as-isolated form. Presence of 0.1 mM enhances enzymic activity by 2fold | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49600 | - |
gel fitlration | Pyrobaculum aerophilum |
52000 | - |
1 * 52900, calculated, 1 * 52000, SDS-PAGE | Pyrobaculum aerophilum |
52900 | - |
1 * 52900, calculated, 1 * 52000, SDS-PAGE | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme shows Cu+/Fe2+ oxidation kinetics that follow the Michaelis-Menten model, with 2fold to 10fold higher efficiencies for Cu+ and Fe2+ as compared with the tested aromatic compounds | Pyrobaculum aerophilum | ? | - |
? | |
N2O + 2 Fe2+ + 2 H+ | - |
Pyrobaculum aerophilum | N2 + H2O + 2 Fe3+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52900, calculated, 1 * 52000, SDS-PAGE | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
McoP | - |
Pyrobaculum aerophilum |
multicopper oxidase | - |
Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life 5.5 h | Pyrobaculum aerophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
N2O | pH 7.6, 25°C | Pyrobaculum aerophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
242 | - |
Fe2+ | pH 7.6, 25°C | Pyrobaculum aerophilum | |
250 | - |
N2O | pH 7.6, 25°C | Pyrobaculum aerophilum |