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Literature summary for 1.7.2.5 extracted from

  • Kaya, M.; Matsumura, K.; Higashida, K.; Hata, Y.; Kawato, A.; Abe, Y.; Akita, O.; Takaya, N.; Shoun, H.
    Cloning and enhanced expression of the cytochrome P450nor gene (nicA; CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae (2004), Biosci. Biotechnol. Biochem., 68, 2040-2049.
    View publication on PubMed
Search for more literature for 1.7.2.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Aspergillus oryzae Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 NO 37°C, pH 7.2 Aspergillus oryzae
0.8
-
 NADH 37°C, pH 7.2 Aspergillus oryzae
0.93
-
 NADPH 37°C, pH 7.2 Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Aspergillus oryzae
45689
-
 x * 45689, deduced from nucleotide sequence Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NO + NADH Aspergillus oryzae
-
 N2O + H2O + NAD+
-
 ?
NO + NADH Aspergillus oryzae OSI 1013
-
 N2O + H2O + NAD+
-
 ?
NO + NADPH Aspergillus oryzae
-
 N2O + H2O + NADP+
-
 ?
NO + NADPH Aspergillus oryzae OSI 1013
-
 N2O + H2O + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q8NKB4
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Resource Q, Resource PHE, Superdex 200 Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NO + NADH
-
 Aspergillus oryzae N2O + H2O + NAD+
-
 ?
NO + NADH NADH is preferred over NADPH Aspergillus oryzae N2O + H2O + NAD+
-
 ?
NO + NADH
-
 Aspergillus oryzae OSI 1013 N2O + H2O + NAD+
-
 ?
NO + NADH NADH is preferred over NADPH Aspergillus oryzae OSI 1013 N2O + H2O + NAD+
-
 ?
NO + NADPH
-
 Aspergillus oryzae N2O + H2O + NADP+
-
 ?
NO + NADPH
-
 Aspergillus oryzae OSI 1013 N2O + H2O + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Aspergillus oryzae
? x * 45689, deduced from nucleotide sequence Aspergillus oryzae

Synonyms

Synonyms Comment Organism
Anor
-
 Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
166.7
-
 NO 37°C, PH 7.2 Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Aspergillus oryzae
NADPH
-
 Aspergillus oryzae