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Literature summary for 1.7.2.5 extracted from

  • Thorndycroft, F.H.; Butland, G.; Richardson, D.J.; Watmough, N.J.
    A new assay for nitric oxide reductase reveals two conserved glutamate residues form the entrance to a proton-conducting channel in the bacterial enzyme (2007), Biochem. J., 401, 111-119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Paracoccus denitrificans

Protein Variants

Protein Variants Comment Organism
E122A substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
E122D substitution mutant retains 83.2% of the activity of the wild-type enzyme Paracoccus denitrificans
E122Q substitution mutant retains 1.3% of the activity of the wild-type enzyme Paracoccus denitrificans
E125A substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
E125D substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
E125Q substitution mutant retains 13.4% of the activity of the wild-type enzyme Paracoccus denitrificans
E198A substitution mutant retains 1.5% of the activity of the wild-type enzyme Paracoccus denitrificans
E202A substitution mutant retains 39.3% of the activity of the wild-type enzyme Paracoccus denitrificans
E267A substitution mutant retains 3.6% of the activity of the wild-type enzyme Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
pseudoazurin apparent KM of native enzyme Paracoccus denitrificans
0.035
-
pseudoazurin apparent KM of recombinant enzyme Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Paracoccus denitrificans 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitric oxide + reduced pseudoazurin Paracoccus denitrificans
-
nitrous oxide + pseudoazurin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 nitric oxide + reduced acceptor
-
Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
?
nitric oxide + reduced pseudoazurin
-
Paracoccus denitrificans nitrous oxide + pseudoazurin + H2O
-
?

Synonyms

Synonyms Comment Organism
nitric oxide reductase
-
Paracoccus denitrificans
NO reductase
-
Paracoccus denitrificans
NOR
-
Paracoccus denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.88
-
pseudoazurin mutant E122Q Paracoccus denitrificans
1.01
-
pseudoazurin mutant E198A Paracoccus denitrificans
1.27
-
pseudoazurin mutant E125D Paracoccus denitrificans
1.3
-
pseudoazurin recombinant wild type enzyme Paracoccus denitrificans
2.58
-
pseudoazurin mutant E267A Paracoccus denitrificans
5.18
-
pseudoazurin mutant E125A Paracoccus denitrificans
7
-
pseudoazurin mutant E122D Paracoccus denitrificans
9.43
-
pseudoazurin mutant E125Q Paracoccus denitrificans
10.2
-
pseudoazurin mutant E122A Paracoccus denitrificans
15
-
pseudoazurin native wild type enzyme Paracoccus denitrificans
27.67
-
pseudoazurin mutant E202A Paracoccus denitrificans
58.52
-
pseudoazurin mutant E122D Paracoccus denitrificans
70.38
-
pseudoazurin recombinant wild type enzyme Paracoccus denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Paracoccus denitrificans