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Literature summary for 1.7.2.5 extracted from

  • Field, S.J.; Thorndycroft, F.H.; Matorin, A.D.; Richardson, D.J.; Watmough, N.J.
    The respiratory nitric oxide reductase (NorBC) from Paracoccus denitrificans (2008), Methods Enzymol., 437, 79-101.
    View publication on PubMed
Search for more literature for 1.7.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane NorBC is a complex integral membrane protein Paracoccus denitrificans 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 1 * 56000 + 1 * 17000 Paracoccus denitrificans
56000
-
 1 * 56000 + 1 * 17000 Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-
Paracoccus denitrificans 93.11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and HiTrap chelating Sepharose column chromatography Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitric oxide + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Paracoccus denitrificans nitrous oxide + oxidized N,N,N',N'-tetramethyl-p-phenylenediamine + H2O
-
 ?
nitric oxide + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized N,N,N',N'-tetramethyl-p-phenylenediamine + H2O
-
 ?
nitric oxide + reduced phenazine methosulfate
-
 Paracoccus denitrificans nitrous oxide + oxidized phenazine methosulfate + H2O
-
 ?
nitric oxide + reduced phenazine methosulfate
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized phenazine methosulfate + H2O
-
 ?
nitric oxide + reduced pseudoazurin
-
 Paracoccus denitrificans nitrous oxide + oxidized pseudoazurin + H2O
-
 ?
nitric oxide + reduced pseudoazurin
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized pseudoazurin + H2O
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 56000 + 1 * 17000 Paracoccus denitrificans

Synonyms

Synonyms Comment Organism
NorBC
-
 Paracoccus denitrificans
respiratory nitric oxide reductase
-
 Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
cytochrome NorBC is a two subunit cytochrome bc complex Paracoccus denitrificans
heme subunit NorB contains a magnetically isolated low-spin heme b Paracoccus denitrificans