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Literature summary for 1.7.2.5 extracted from
- Camelid nanobodies raised against an integral membrane enzyme, nitric oxide reductase (2009), Protein Sci., 18, 619-628.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with variable heavy chain domain of camel heavy chain antibody, sitting drop vapor diffusion method, using 100 mM potassium phosphate pH 7.0, 0.01% (v/v) phenyl ethanol, 0.005% (v/v) n-dodecyl-beta-D-maltopyranoside | Marinobacter nauticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral membrane enzyme | Marinobacter nauticus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17600 | - |
subunit NorC, gel filtration, not detectable by mass spectrometry | Marinobacter nauticus |
| 38000 | - |
subunit NorB, gel filtration, not detectable by mass spectrometry | Marinobacter nauticus |
| 54400 | - |
subunit NorB, calculated from amino acid sequence | Marinobacter nauticus |
| 71800 | - |
NOR enzyme complex, calculated from amino acid sequence | Marinobacter nauticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Marinobacter nauticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography and hydroxyapatite column chromatography | Marinobacter nauticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitric oxide + ferrocytochrome c552 | - |
Marinobacter nauticus | nitrous oxide + ferricytochrome c552 + H2O | - |
? |  |
| nitric oxide + reduced acceptor | - |
Marinobacter nauticus | nitrous oxide + oxidized acceptor + H2O | - |
? | |
| nitric oxide + reduced ascorbate | - |
Marinobacter nauticus | nitrous oxide + oxidized ascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitric oxide reductase | - |
Marinobacter nauticus |
| NOR | - |
Marinobacter nauticus |
| NorB | subunit | Marinobacter nauticus |
| NorC | subunit | Marinobacter nauticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Marinobacter nauticus | |
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Release 2023.1 (January 2023)
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