Protein Variants | Comment | Organism |
---|---|---|
C397S | the mutation results in decreases in the rate constants for removal of the substrate proton by about 5fold and decreases in the rate constant for product release of about 2fold, the mutant enzyme is less stable than the wild type enzyme | Fusarium oxysporum |
S171A | the mutation results in decreases in the rate constants for removal of the substrate proton by about 5fold and decreases in the rate constant for product release of about 2fold | Fusarium oxysporum |
S171T | the mutation results in decreases in the rate constants for removal of the substrate proton by about 5fold and decreases in the rate constant for product release of about 2fold, the mutation alters the rate constant for flavin oxidation | Fusarium oxysporum |
S171V | the mutation results in decreases in the rate constants for removal of the substrate proton by about 5fold and decreases in the rate constant for product release of about 2fold, the mutation alters the rate constant for flavin oxidation | Fusarium oxysporum |
Y398F | the mutation results in decreases in the rate constants for removal of the substrate proton by about 5fold and decreases in the rate constant for product release of about 2fold, the mutant enzyme is less stable than the wild type enzyme | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
O2 | mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
0.028 | - |
O2 | mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
0.029 | - |
O2 | mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
0.044 | - |
O2 | mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
0.058 | - |
O2 | mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
0.082 | - |
O2 | wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
1.9 | - |
nitroethane | mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
2 | - |
nitroethane | mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
2 | - |
nitroethane | mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
2.1 | - |
nitroethane | mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
2.3 | - |
nitroethane | wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
4.8 | - |
nitroethane | mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitroethane + O2 + H2O | - |
Fusarium oxysporum | ethanal + nitrite + H2O2 + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nitroethane oxidase | - |
Fusarium oxysporum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
nitroethane | mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
6.2 | - |
nitroethane | mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
7.2 | - |
nitroethane | mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
7.3 | - |
nitroethane | mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
10.6 | - |
nitroethane | mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
15 | - |
nitroethane | wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.1 mM FAD is used in assay conditions | Fusarium oxysporum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
nitroethane | mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
2.6 | - |
nitroethane | mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
3.2 | - |
nitroethane | mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
3.6 | - |
nitroethane | mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
6.3 | - |
nitroethane | wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
140 | - |
O2 | mutant enzyme S171V, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
190 | - |
O2 | mutant enzyme S171A, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
290 | - |
O2 | mutant enzyme C397S, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
310 | - |
O2 | wild type enzyme, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
390 | - |
O2 | mutant enzyme Y398F, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum | |
580 | - |
O2 | mutant enzyme S171T, in 200 mM HEPES, 0.1 mM FAD, pH 8.0, 30 °C | Fusarium oxysporum |