Crystallization (Comment) | Organism |
---|---|
wild-type and mutant H179D. Enzyme consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a alpha-alpha-alpha-beta-alpha-beta-alpha fold. It shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase | Streptomyces ansochromogenes |
Protein Variants | Comment | Organism |
---|---|---|
H179D | residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity | Streptomyces ansochromogenes |
H179K | residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity | Streptomyces ansochromogenes |
H179V | residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity | Streptomyces ansochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.8 | - |
nitroethane | pH 8.0, 28°C | Streptomyces ansochromogenes | |
35.7 | - |
1-Nitropropane | pH 8.0, 28°C | Streptomyces ansochromogenes | |
83.5 | - |
2-Nitropropane | pH 8.0, 28°C | Streptomyces ansochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces ansochromogenes | Q9FDD4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-nitropropane + H2O | - |
Streptomyces ansochromogenes | propanal + nitrite + H2O2 | - |
? | |
2-nitropropane + H2O | - |
Streptomyces ansochromogenes | propanone + nitrite + H2O2 | - |
? | |
additional information | enzyme prefers the neutral forms of substrates and only reacts with anionic 2-nitropropane at a low rate | Streptomyces ansochromogenes | ? | - |
? | |
nitroethane + H2O | - |
Streptomyces ansochromogenes | ethanal + nitrite + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization data | Streptomyces ansochromogenes |
Synonyms | Comment | Organism |
---|---|---|
2-npdl | - |
Streptomyces ansochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Streptomyces ansochromogenes |