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Literature summary for 1.7.3.1 extracted from

  • Li, Y.; Gao, Z.; Hou, H.; Li, L.; Zhang, J.; Yang, H.; Dong, Y.; Tan, H.
    Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes (2011), Biochem. Biophys. Res. Commun., 405, 344-348.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutant H179D. Enzyme consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a alpha-alpha-alpha-beta-alpha-beta-alpha fold. It shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase Streptomyces ansochromogenes

Protein Variants

Protein Variants Comment Organism
H179D residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity Streptomyces ansochromogenes
H179K residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity Streptomyces ansochromogenes
H179V residue is spatially adjacent to FMN, mutation results in the loss of enzyme activity Streptomyces ansochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26.8
-
nitroethane pH 8.0, 28°C Streptomyces ansochromogenes
35.7
-
1-Nitropropane pH 8.0, 28°C Streptomyces ansochromogenes
83.5
-
2-Nitropropane pH 8.0, 28°C Streptomyces ansochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces ansochromogenes Q9FDD4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitropropane + H2O
-
Streptomyces ansochromogenes propanal + nitrite + H2O2
-
?
2-nitropropane + H2O
-
Streptomyces ansochromogenes propanone + nitrite + H2O2
-
?
additional information enzyme prefers the neutral forms of substrates and only reacts with anionic 2-nitropropane at a low rate Streptomyces ansochromogenes ?
-
?
nitroethane + H2O
-
Streptomyces ansochromogenes ethanal + nitrite + H2O2
-
?

Subunits

Subunits Comment Organism
dimer crystallization data Streptomyces ansochromogenes

Synonyms

Synonyms Comment Organism
2-npdl
-
Streptomyces ansochromogenes

Cofactor

Cofactor Comment Organism Structure
FMN
-
Streptomyces ansochromogenes