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Literature summary for 1.7.3.3 extracted from

  • Nishiya, Y.; Hibi, T.; Oda, J.
    A purification method of the diagnostic enzyme Bacillus uricase using magnetic beads and non-specific protease (2002), Protein Expr. Purif., 25, 426.
    View publication on PubMed
Search for more literature for 1.7.3.3

Application

Application Comment Organism
medicine determining the urate concentration in blood and urine Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli JM109 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 uric acid enzyme without histidine tag Bacillus sp. (in: Bacteria)
0.015
-
 uric acid enzyme with histidine tag Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Bacillus sp. (in: Bacteria)
-
 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O Bacillus sp. (in: Bacteria) TB-90
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 TB-90
-
Bacillus sp. (in: Bacteria) TB-90
-
 TB-90
-

Purification (Commentary)

Purification (Comment) Organism
enzyme synthesized with C-terminal 6-histidine tag Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.29
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Bacillus sp. (in: Bacteria) 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O
-
 Bacillus sp. (in: Bacteria) TB-90 5-hydroxyisourate + H2O2
-
 ?