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Literature summary for 1.7.3.3 extracted from

  • Liu, Z.; Lu, D.; Li, J.; Chen, W.; Liu, Z.
    Strengthening intersubunit hydrogen bonds for enhanced stability of recombinant urate oxidase from Aspergillus flavus: molecular simulations and experimental validation (2009), Phys. Chem. Chem. Phys., 11, 333-340.
    View publication on PubMed

Application

Application Comment Organism
medicine substitute for allopurinol in the management of gout and hyperuricaemia Aspergillus flavus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Aspergillus flavus first step in uric acid degradation 5-hydroxyisourate + H2O2
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO the residual recombinant UOX activity in the presence of DMSO is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the DMSO concentration up to 20%, further increase in DMSO concentration (50-70%) results in significant UOX deactivation Aspergillus flavus
Methanol the residual recombinant UOX activity in the presence of methanol is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the methanol concentration up to 20%, further increase in methanol concentration (50-70%) results in significant UOX deactivation Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O first step in uric acid degradation Aspergillus flavus 5-hydroxyisourate + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer determined by gel filtration, tunnel-shaped homotetramer, each of the 4 active sites in UOX is formed by residues from 2 subunits and located at the subunit-subunit interface Aspergillus flavus

Synonyms

Synonyms Comment Organism
Rasburicase commercially available recombinant enzyme Aspergillus flavus
Uox
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Aspergillus flavus
Urate oxidase
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Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal deactivation of recombinant UOX at neutral pH is associated with the loss of intersubunit hydrogen bonds, subunit is unstable at room temperature and unfolds rapidly at 100°C, tetramer has significantly higher stability than its subunit Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
assay at Aspergillus flavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml Aspergillus flavus