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Literature summary for 1.7.3.3 extracted from

  • Pfrimer, P.; de Moraes, L.M.; Galdino, A.S.; Salles, L.P.; Reis, V.C.; De Marco, J.L.; Prates, M.V.; Bloch, C.; Torres, F.A.
    Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli (2010), J. Biomed. Biotechnol., 2010, 674908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58680
-
MALDI-TOF mass spectrometry Bacillus subtilis
58900
-
calculated from amino acid sequence Bacillus subtilis
60000
-
about 60000 Da, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
subspecies subtilis LMD 69.3
-
Bacillus subtilis LMD 69.3
-
subspecies subtilis LMD 69.3
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
crude extract, pH 8.0, 37°C Bacillus subtilis
39
-
after 2.1fold purification, pH 8.0, 37°C Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 72 h, 20% loss of activity Bacillus subtilis
37°C, purified enzyme, 12 h and 48 h, 50% and 100% loss of activity Bacillus subtilis
4°C, purified enzyme, 72 h, 20% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
Bacillus subtilis 5-hydroxyisourate + H2O2
-
?
urate + O2 + H2O
-
Bacillus subtilis LMD 69.3 5-hydroxyisourate + H2O2
-
?

Synonyms

Synonyms Comment Organism
Urate oxidase
-
Bacillus subtilis
uricase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 80 about 80% relative activity at 10°C, about 85% relative activity at 20-30°C, about 90% relative activity at 37°C, about 40% relative activity at 40°C, about 30% relative activity at 50°C, about 20% relative activity at 60°C, about 10% relative activity at 70°C, and no activity at 80°C after 30 min incubation Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Bacillus subtilis