Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58680 | - |
MALDI-TOF mass spectrometry | Bacillus subtilis |
58900 | - |
calculated from amino acid sequence | Bacillus subtilis |
60000 | - |
about 60000 Da, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
subspecies subtilis LMD 69.3 | - |
Bacillus subtilis LMD 69.3 | - |
subspecies subtilis LMD 69.3 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
crude extract, pH 8.0, 37°C | Bacillus subtilis |
39 | - |
after 2.1fold purification, pH 8.0, 37°C | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 72 h, 20% loss of activity | Bacillus subtilis |
37°C, purified enzyme, 12 h and 48 h, 50% and 100% loss of activity | Bacillus subtilis |
4°C, purified enzyme, 72 h, 20% loss of activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Bacillus subtilis | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Bacillus subtilis LMD 69.3 | 5-hydroxyisourate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Urate oxidase | - |
Bacillus subtilis |
uricase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | about 80% relative activity at 10°C, about 85% relative activity at 20-30°C, about 90% relative activity at 37°C, about 40% relative activity at 40°C, about 30% relative activity at 50°C, about 20% relative activity at 60°C, about 10% relative activity at 70°C, and no activity at 80°C after 30 min incubation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Bacillus subtilis |