Activating Compound | Comment | Organism | Structure |
---|---|---|---|
propane-2-nitronate | increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase | Penicillium atrovenetum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Penicillium atrovenetum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE in presence of mercaptoethanol | Penicillium atrovenetum |
73000 | - |
gel filtration | Penicillium atrovenetum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium atrovenetum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Penicillium atrovenetum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2 | primary products of the enzymatic reaction probably is the nitropropanoate free radical and superoxide | Penicillium atrovenetum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium atrovenetum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
butyrate-4-nitronate as substrate | Penicillium atrovenetum |
274 | - |
propionate-3-nitronate as substrate | Penicillium atrovenetum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aci-nitropropanoate + O2 | - |
Penicillium atrovenetum | 3-oxopropanoate + nitrite + H2O2 | - |
? | |
butyrate-4-nitronate + O2 | - |
Penicillium atrovenetum | succinate + nitrite + H2O2 | - |
? | |
additional information | neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate | Penicillium atrovenetum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE in presence of mercaptoethanol | Penicillium atrovenetum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Penicillium atrovenetum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
350 | - |
3-aci-Nitropropanoate | - |
Penicillium atrovenetum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Penicillium atrovenetum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein | Penicillium atrovenetum |