Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | the cyanide-sensitive form of the enzyme exists only during turnover | Paracoccus denitrificans | |
EDTA | 1 mM, in 10 mM Tris/HCl, pH 8.0, almost complete inhibition | Paracoccus denitrificans | |
NaCl | 15 mM, 50% inhibition of activity with horse heart cytochrome, activity with pseudoazurin or cytochrome c550 is inhibited only above 100 mM | Paracoccus denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 3-5 nonhaem, non-iron-sulfur iron atoms as prosthetic groups, predominantly coordinated by carboxylate ligands | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
strain GB17, heterotrophic nitrifier | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + ferricytochrome c550 | under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction | Paracoccus denitrificans | nitroxyl + ferrocytochrome c550 + H+ | - |
? | |
hydroxylamine + O2 | horse heart cytochrome. Under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction | Paracoccus denitrificans | nitrite + H2O + H+ | - |
? | |
hydroxylamine + pseudoazurin | under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction | Paracoccus denitrificans | nitrite + reduced pseudoazurin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hydroxylamine oxidase | - |
Paracoccus denitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
hydroxylamine | pH 8, temperature not specified in the publication | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity with horse heart cytochrome c | Paracoccus denitrificans |
10 | - |
activity with pseudoazurin | Paracoccus denitrificans |
11 | - |
activity with cytochrome c550 continues to increase to pH 11 | Paracoccus denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 11.5 | pH 7.3: about 35% of maximal activity, pH 11.5: about 50% of maximal activity activity with pseudoazurin | Paracoccus denitrificans |
7.6 | 10 | pH 7.6: about 50% of maximal activity, pH 10.0: about 35% of maximal activity with horse heart cytochrome c | Paracoccus denitrificans |
8 | 11 | activity with cytochrome c550 continues to increase to pH 11, activity at pH 8 is about 50% of the activity at pH 11 | Paracoccus denitrificans |