Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | slightly inhibitory | Spinacia oleracea | |
cyanide | almost complete inhibition from 0.02 mM to 0.2 mM | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the native enzyme with a MW of 85000 can be split into a modified form of a MW of 61000 that retains activity with the non-physiological electron-donor, methyl viologen but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
301.4 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | spinach: the native enzyme with a molecular weight of 85000 can be split into a modified form with a MW of 61000 that retains activity with the non-physiological electron-donor methyl viologen, but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | prosthetic group | Spinacia oleracea | |
siroheme | 2 mol of siroheme per mol of enzyme | Spinacia oleracea |