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Literature summary for 1.7.7.1 extracted from

  • Hirasawa-Soga, M.; Horie, S.; Tamura, G.
    Further characterization of ferredoxin nitrite reductase and the relationship between the enzyme and methyl viologen-dependent nitrite reductase (1982), Agric. Biol. Chem., 46, 1319-1328.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 slightly inhibitory Spinacia oleracea
cyanide almost complete inhibition from 0.02 mM to 0.2 mM Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the native enzyme with a MW of 85000 can be split into a modified form of a MW of 61000 that retains activity with the non-physiological electron-donor, methyl viologen but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
301.4
-
-
Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced ferredoxin
-
Spinacia oleracea ammonia + oxidized ferredoxin
-
?

Subunits

Subunits Comment Organism
More spinach: the native enzyme with a molecular weight of 85000 can be split into a modified form with a MW of 61000 that retains activity with the non-physiological electron-donor methyl viologen, but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
siroheme prosthetic group Spinacia oleracea
siroheme 2 mol of siroheme per mol of enzyme Spinacia oleracea