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Literature summary for 1.7.7.2 extracted from

  • Martinez-Espinosa, R.M.; Marhuenda-Egea, F.C.; Bonete, M.J.
    Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: Purification and characterization (2001), FEMS Microbiol. Lett., 204, 381-385.
    View publication on PubMed
Search for more literature for 1.7.7.2

General Stability

General Stability Organism
NaCl stabilizes, after 43 h, with 2.7 M, 2.1 M or 0.9 M NaCl: 95% loss of activity, after 90 h, with 4.3 M NaCl: completely stable Haloferax mediterranei

Inhibitors

Inhibitors Comment Organism Structure
azide potent inhibitor, 79% loss of activity Haloferax mediterranei
cyanide almost complete inhibition Haloferax mediterranei
EDTA partial inhibition, 43% loss of activity Haloferax mediterranei
additional information not inhibited by potassium chlorate, dithiothreitol, p-hydroxymercuribenzoate, sulfite Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 reduced methyl viologen
-
 Haloferax mediterranei
0.95
-
 nitrate
-
 Haloferax mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
iron-sulfur centre enzyme contains Fe-S clusters Haloferax mediterranei
NaCl activity depends on salt concentration Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 105000 + 1 * 50000, SDS-PAGE Haloferax mediterranei
105000
-
 1 * 105000 + 1 * 50000, SDS-PAGE Haloferax mediterranei
132000
-
 gel filtration Haloferax mediterranei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + reduced ferredoxin Haloferax mediterranei assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
 extreme halophilic archaeon
-

Purification (Commentary)

Purification (Comment) Organism
177fold purification Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
-
 Haloferax mediterranei

Storage Stability

Storage Stability Organism
4°C, 50 mM phosphate buffer, pH 7, 20% glycerol, 2 weeks, no loss of activity Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not as electron donors: NADH, NADPH, dithionite, 8% of maximum activity with 1mM FADH2 and less than 7% of maximum activity with 1 mM reduced 2,6-dichlorophenolindophenol or phenazine methosulfate Haloferax mediterranei ?
-
 ?
nitrate + reduced ferredoxin 100 nM dithionite reduced ferredoxin with 94% of the activity with reduced methyl viologen Haloferax mediterranei nitrite + H2O + oxidized ferredoxin
-
 ?
nitrate + reduced ferredoxin assimilatory nitrate reduction Haloferax mediterranei nitrite + oxidized ferredoxin
-
 ?
nitrate + reduced methyl viologen reduced methyl viologen is the best electron donor in vitro Haloferax mediterranei nitrite + oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 105000 + 1 * 50000, SDS-PAGE Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 strong dependence of activity on the temperature and NaCl concentrations Haloferax mediterranei
60
-
 for NaCl concentrations of 1.3 and 0.9 M Haloferax mediterranei
80
-
 for NaCl concentrations of 3.1 and 2.2 M Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Haloferax mediterranei

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre enzyme contains Fe-S clusters Haloferax mediterranei
additional information no FAD or cytochromes are observed Haloferax mediterranei