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Literature summary for 1.7.99.1 extracted from
- A thermostable hybrid cluster protein from Pyrococcus furiosus: effects of the loss of a three helix bundle subdomain (2009), J. Biol. Inorg. Chem., 14, 703-710.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
hydroxylamine | purified enzyme, at 70°C and pH 9.0 | Pyrococcus furiosus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains 2.7 Fe per molecule, in the case of full occupation of a hybrid cluster and a [4Fe-4S] cluster 8 Fe/molecule can be expected | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51300 | - |
2 * 51300, calculated from amino acid sequence | Pyrococcus furiosus |
| 52000 | - |
2 * 52000, SDS-PAGE | Pyrococcus furiosus |
| 102000 | - |
native PAGE | Pyrococcus furiosus |
| 102600 | - |
calculated from amino acid sequence | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Poros HQ anion-exchange column chromatography and Superdex 200 gel filtration | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
purified enzyme, at 70°C and pH 9.0 | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + reduced methyl viologen | - |
Pyrococcus furiosus | ammonia + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 52000, SDS-PAGE | Pyrococcus furiosus |
| homodimer | 2 * 51300, calculated from amino acid sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fuscoredoxin | - |
Pyrococcus furiosus |
| HCP | - |
Pyrococcus furiosus |
| hybrid cluster protein | - |
Pyrococcus furiosus |
| prismane protein | - |
Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
hydroxylamine | purified enzyme, at 70°C and pH 9.0 | Pyrococcus furiosus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.5 | - |
hydroxylamine | purified enzyme, at 70°C and pH 9.0 | Pyrococcus furiosus | |
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Release 2023.1 (January 2023)
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