Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lecithin | drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II | Asparagus officinalis | |
NAD+ | reduction of lipoic acid is activated | Asparagus officinalis | |
Tween 80 | drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II | Asparagus officinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | low inhibition | Asparagus officinalis | |
asparagusic acid | inhibits lipoyl dehydrogenase activity | Asparagus officinalis | |
cetyltrimethyl ammonium bromide | affects lipoyl dehydrogenase activity | Asparagus officinalis | |
FAD | strongly inhibits the two reductase activities | Asparagus officinalis | |
Hg2+ | inhibitory | Asparagus officinalis | |
iodoacetamide | low inhibition | Asparagus officinalis | |
additional information | inhibitors indicate an involvement of protein disulfide linkage or thiol group in the catalytic site | Asparagus officinalis | |
N-ethylmaleimide | - |
Asparagus officinalis | |
NADH | high concentrations, increase of inhibitory effect by addition of arsenite | Asparagus officinalis | |
p-chloromercuribenzoic acid | - |
Asparagus officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
potassium ferricyanide | enzyme II | Asparagus officinalis | |
0.9 | - |
potassium ferricyanide | enzyme I | Asparagus officinalis | |
3 | - |
lipoic acid | - |
Asparagus officinalis | |
3 | 3.3 | lipoate | enzyme I and II | Asparagus officinalis | |
20 | - |
asparagusic acid | - |
Asparagus officinalis | |
20 | 21.5 | Asparagusate | enzyme I and II | Asparagus officinalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Asparagus officinalis | 5739 | - |
soluble | - |
Asparagus officinalis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | 111000 | ultracentrifugation | Asparagus officinalis |
112000 | - |
calculation of amino acid content | Asparagus officinalis |
112000 | - |
enzyme I and II, gel filtration | Asparagus officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
asparagusate + NADH | Asparagus officinalis | - |
dihydroasparagusate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Asparagus officinalis | - |
2 asparagusate dehydrogenases with lipoyl dehydrogenase activity | - |
Purification (Comment) | Organism |
---|---|
gel filtration, ion-exchange, ultracentrifugation, copurification of type I and II | Asparagus officinalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ | exhibits lipoyl dehydrogenase activity | Asparagus officinalis | |
3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ | exhibits diaphorase activity | Asparagus officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Asparagus officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.215 | - |
enzyme/fraction II towards asparagusic acid | Asparagus officinalis |
0.425 | - |
enzyme/fraction I towards asparagusic acid | Asparagus officinalis |
0.5464 | - |
enzyme/fraction II towards lipoic acid | Asparagus officinalis |
1.082 | - |
enzyme/fraction I towards lipoic acid | Asparagus officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Asparagus officinalis | 2 ferrocyanide + NAD+ + H+ | - |
? | |
asparagusate + NADH | - |
Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
asparagusic acid + NADH | reverse reaction very slow | Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
lipoic acid + NADH | reverse reaction very slow | Asparagus officinalis | dihydrolipoic acid + NAD+ | - |
r | |
NADH + H+ + potassium ferricyanide | - |
Asparagus officinalis | NAD+ + potassium ferrocyanide | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Asparagus officinalis |
40 | 45 | - |
Asparagus officinalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, activity completely retained | Asparagus officinalis |
50 | - |
above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C | Asparagus officinalis |
60 | 70 | 5 min, 1.4-fold activation of asparagusate dehydrogenase activity | Asparagus officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.25 | - |
for K3Fe(CN)6 reduction | Asparagus officinalis |
5.9 | - |
asparagusic and lipoic acid reduction | Asparagus officinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, approximately 1 mol of FAD per mol of protein | Asparagus officinalis | |
NAD+ | up to 0.2 mM, stimulates the reduction activity if NADH concentration is 0.2 mM | Asparagus officinalis | |
NADH | no replacement of NADH by NADPH | Asparagus officinalis | |
NADH | 0.1 mM optimal concentration for maximal activity | Asparagus officinalis |