Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-Bis(2-chloroethyl)-1-nitrosourea | - |
Trypanosoma cruzi | |
2,3-Bis(3-(2-amidinohydrazono)-butyl)-1,4-naphthoquinone dihydrochloride | - |
Trypanosoma cruzi | |
2-(5-nitro-2-furanylmethylidene)-N,N'-(1,4-piperazinediylbis(1,3-propanediyl))bishydrazinecarboximidamide tetrahydrobromide | - |
Trypanosoma cruzi | |
additional information | increasing ionic strength leads to inhibition, e.g. with NaCl, KCl, (NH4)SO4, HEPES | Trypanosoma cruzi | |
nifurtimox | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | 0.007 | NADPH | - |
Trypanosoma cruzi | |
0.018 | 0.14 | trypanothione disulfide | dependent on buffer system | Trypanosoma cruzi | |
0.022 | 0.275 | glutathionylspermidine disulfide | dependent on buffer system | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
r | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
glutathionylspermidine disulfide + NADPH, additional 2 minor optima at pH 7.0 and pH 8.0 | Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
nifurtimox | - |
Trypanosoma cruzi |