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Literature summary for 1.8.1.12 extracted from

  • Krauth-Siegel, R.L.; Enders, B.; Henderson, G.B.; Fairlamb, A.H.; Schirmer, R.H.
    Trypanothione reductase from Trypanosoma cruzi. Purification and characterization of the crystalline enzyme (1987), Eur. J. Biochem., 164, 123-128.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method in presence of 2 M (NH4)2SO4 at 4°C Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide inhibition only of the enzyme with reduced cysteine residues in the catalytic center by alkylation Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km dependent on assay conditions Trypanosoma cruzi
0.005 0.007 NADPH
-
Trypanosoma cruzi
0.022 0.275 glutathionylspermidine disulfide
-
Trypanosoma cruzi
0.045
-
trypanothione disulfide
-
Trypanosoma cruzi
0.275
-
glutathionylspermidine disulfide
-
Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Trypanosoma cruzi
100000
-
gel filtration Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trypanosoma cruzi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
284
-
purified enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathionylspermidine disulfide + NADPH
-
Trypanosoma cruzi 2-glutathionylspermidine + NADP+
-
r
trypanothione disulfide + NADPH
-
Trypanosoma cruzi trypanothione + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
237
-
trypanothione disulfide dependent on assay conditions Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trypanosoma cruzi
NADH low activity with NADH Trypanosoma cruzi
NADPH enzyme also utilizes NADH, but less efficiently Trypanosoma cruzi