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Literature summary for 1.8.1.12 extracted from

  • Shames, S.L.; Fairlamb, A.H.; Cerami, A.; Walsh, C.T.
    Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the family of disulfide-containing flavoprotein reductases (1986), Biochemistry, 25, 3519-3526.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Crithidia fasciculata

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide inhibition only of the enzyme with reduced cysteine residues in the catalytic center by alkylation Crithidia fasciculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005 0.007 NADPH
-
Crithidia fasciculata
0.053
-
trypanothione disulfide
-
Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53800
-
1 * 53800, SDS-PAGE Crithidia fasciculata
110000
-
enzyme cross-linked with dimethyl suberimidate, SDS-PAGE Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypanothione disulfide + NADPH Crithidia fasciculata
-
trypanothione + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
insect-parasitic trypanosomatid
-

Purification (Commentary)

Purification (Comment) Organism
-
Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
284
-
purified enzyme Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trypanothione disulfide + NADPH
-
Crithidia fasciculata trypanothione + NADP+
-
?
trypanothione disulfide + NADPH highly specific for Crithidia fasciculata trypanothione + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 1 * 53800, SDS-PAGE Crithidia fasciculata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
517
-
trypanothione disulfide
-
Crithidia fasciculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 trypanothione disulfide + NADPH Crithidia fasciculata

Cofactor

Cofactor Comment Organism Structure
FAD
-
Crithidia fasciculata
NADPH
-
Crithidia fasciculata