Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3)pLysS cells | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M bis-Tris propane pH 8.0, 5% (v/v) PEG 400, 2M ammonium sulfate | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(3,4-dichlorophenyl)-3-(dimethylamino)propan-1-one | - |
Trypanosoma brucei | |
3-(dimethylamino)-1-(3-nitrophenyl)propan-1-one | - |
Trypanosoma brucei | |
3-(dimethylamino)-1-[4-(methylsulfonyl)phenyl]propan-1-one | - |
Trypanosoma brucei | |
4-[[bis(2-hydroxyethyl)amino]methyl]-2,6-dibutylphenol | - |
Trypanosoma brucei | |
citalopram | - |
Trypanosoma brucei | |
clomipramine | - |
Trypanosoma brucei | |
ebselen | - |
Trypanosoma brucei | |
Quinacrine | - |
Trypanosoma brucei | |
thioridazine | - |
Trypanosoma brucei | |
Trifluoperazine | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
NADPH | in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | |
0.0069 | - |
trypanothione disulfide | in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, gel filtration | Trypanosoma brucei |
53160 | - |
subunit, calculated from amino acid sequence | Trypanosoma brucei |
93000 | - |
analytical ultracentrifugation | Trypanosoma brucei |
96000 | - |
gel filtration | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | P39051 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme is purified by ammonium sulfate precipitation, 2'5'-ADP Sepharose affinity column chromatography, and anion exchange chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the rate of reduction of 0.05 mM glutathione disulfide is 0.03% of that obtained with 0.05 mM trypanothione disulfide | Trypanosoma brucei | ? | - |
? | |
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid) | - |
Trypanosoma brucei | trypanothione disulfide + thionitrobenzoate | - |
? | |
trypanothione disulfide + NADPH + H+ | highly specific for trypanothione disulfide | Trypanosoma brucei | trypanothione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48000, gel filtration | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | - |
Trypanosoma brucei |
TryR | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.8 | - |
trypanothione disulfide | in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trypanosoma brucei | |
NADPH | highly specific for NADPH, activity with 0.15 mM NADH is only 5.7% of that obtained with 0.15 mM NADPH | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00018 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | ebselen | |
0.00958 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | thioridazine | |
0.0111 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | clomipramine | |
0.0221 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | Quinacrine | |
0.0405 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | Trifluoperazine | |
0.0823 | - |
in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei | citalopram |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6782 | - |
trypanothione disulfide | in 40 mM HEPES, pH 7.4, 1 mM EDTA | Trypanosoma brucei |